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Plot the Guide Strand with different optional seeds

Usage

plot_seeds(guide.seq)

Arguments

guide.seq

Guide a.k.a anti-sense sequence oriented 5' > 3'. Sequence must be greater than 8 bp.

Value

A msaggplot of the guide sequence in addition to the available seed sequences

Examples

library(msa)
#> Loading required package: Biostrings
#> Loading required package: BiocGenerics
#> 
#> Attaching package: ‘BiocGenerics’
#> The following objects are masked from ‘package:stats’:
#> 
#>     IQR, mad, sd, var, xtabs
#> The following objects are masked from ‘package:base’:
#> 
#>     Filter, Find, Map, Position, Reduce, anyDuplicated, aperm, append,
#>     as.data.frame, basename, cbind, colnames, dirname, do.call,
#>     duplicated, eval, evalq, get, grep, grepl, intersect, is.unsorted,
#>     lapply, mapply, match, mget, order, paste, pmax, pmax.int, pmin,
#>     pmin.int, rank, rbind, rownames, sapply, setdiff, sort, table,
#>     tapply, union, unique, unsplit, which.max, which.min
#> Loading required package: S4Vectors
#> Loading required package: stats4
#> 
#> Attaching package: ‘S4Vectors’
#> The following object is masked from ‘package:utils’:
#> 
#>     findMatches
#> The following objects are masked from ‘package:base’:
#> 
#>     I, expand.grid, unname
#> Loading required package: IRanges
#> Loading required package: XVector
#> Loading required package: GenomeInfoDb
#> 
#> Attaching package: ‘Biostrings’
#> The following object is masked from ‘package:base’:
#> 
#>     strsplit

# Ttr siRNA sequence
guide.seq = "UUAUAGAGCAAGAACACUGUUUU"

# generate seed plot
plotted.seeds = plot_seeds(guide.seq)
#> use default substitution matrix
#> Registered S3 methods overwritten by 'ggalt':
#>   method                  from   
#>   grid.draw.absoluteGrob  ggplot2
#>   grobHeight.absoluteGrob ggplot2
#>   grobWidth.absoluteGrob  ggplot2
#>   grobX.absoluteGrob      ggplot2
#>   grobY.absoluteGrob      ggplot2
#> Scale for x is already present.
#> Adding another scale for x, which will replace the existing scale.